Coding

Part:BBa_K2220000:Design

Designed by: Nan Rong   Group: iGEM17_BNU-China   (2017-07-16)


Alpha-tubulin Mus musculus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1373
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 381
    Illegal EcoRI site found at 779
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 381
    Illegal EcoRI site found at 779
    Illegal SpeI site found at 1374
    Illegal PstI site found at 1388
    Illegal NotI site found at 7
    Illegal NotI site found at 1381
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 381
    Illegal EcoRI site found at 779
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1374
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 381
    Illegal EcoRI site found at 779
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 381
    Illegal EcoRI site found at 779
    Illegal SpeI site found at 1374
    Illegal PstI site found at 1388
    Illegal AgeI site found at 407
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1116
    Illegal SapI site found at 274


Design Notes

We use S.cerevisiae to express it.


Source

https://www.ncbi.nlm.nih.gov/gene/22142

References